“Lab 3 Structure & Microscopy BIO250L”

Student Name: Click here to enter text.

Access Code (located on the underside of the lid of your lab kit): Click here to enter text.

“Pre-Lab Questions”

1. What determines if a bacterial cell is Gram-positive or Gram-negative? Click here to enter text.

2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe one other type of acellular organism. What characteristics about this organism classify it as acellular? Click here to enter text.

3. Bacteria have many different shapes that often determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies.

4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sources in your answer.) Click here to enter text.

5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any antibiotic treatments? (Be specific.) Click here to enter text.

“EXPERIMENT 1: Staining

Data Tables
Table 1: Experiment 1 Staining Observations

Stain Used:

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Observations:

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Post-Lab Questions
1. How does crystal violet enhance the visualization of microbial features? Click here to enter text.

2. What are some of the limitations of simple staining? Click here to enter text.

3. Give an example of a situation in a lab or medical setting in which simple staining would be utilized. Click here to enter text.