1. How many types of RNA polymerases exist in eukaryotes and what are their locations and functions?

2. How mammalian RNA polymerases recognize the promoter region and what is TATA box initiator element and their functions?

3. What are general transcription factors with their individual function? Explain the preinitiation complex formation?

4. What is posttranscriptional modification of RNA for RNA to be functional? Explain the eukaryotic mRNA 5’ cap and poly A tail formation?

5. How splicing of introns from genes forms the functional mRNA of ovalbumin?