IntroductionRecombinant DNA is the joining together of DNA molecules from two different molecules that are inserted into a host organism to make a new genetic combination. They are used in science,  medicine,  agriculture, and industry. Recombinant DNA was first brought out in 1980, it has helped a lot in the advancement of science. DNA recombination involves the process of cutting and joining DNA of different species through restriction enzymes and ligations (2).The goal of this method is to have an antibiotic resistance gene using recombinant DNA. When it’s inserted into a host cell, it allows the bacteria to grow. The antibiotic gene is formulated from two relaxed plasmid reagents, pKAN and pUC19, each of which contains a single antibiotic resistance gene. These plasmids are digested in a pre-constructed hybrid restriction enzyme mixtures of BamHI and HindIII (3).Each plasmid has a sequence where each enzyme cuts, making two restriction fragments which are produced and analyzed through a process called electrophoresis. Electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size through the passing of electric current (1). The cutting ability of hybrid restriction enzymes is confirmed and enzyme fragments are separated from the DNA fragments through electrophoresis.On the other hand, ligation is a method that requires the presence of ATP and the ends of the fragments are fused by ligation (1). These ligations occur by hydrogen and phosphodiester bonds, which is what forms the recombinant DNA molecule. This recombinant DNA then goes through transformation with E. coli. Transformation is done in order to be able to select the antibiotic resistant gene. The colonies on the plates show how successful the transformation was and if the resistance was accomplished. X-Gal is used in the transformation process for identification purposes. It is a blue/white screening technique that allows for indication of whether a cell expresses a functional B-galactosidase enzyme in blue/white screening. The phenotype is then identified by conducting a purification procedure (4).Materials and MethodsMaterials